eclipse ti-e epifluorescence live-imaging microscope Search Results


99
Sartorius AG incucyte s3 epifluorescence time lapse microscope
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Incucyte S3 Epifluorescence Time Lapse Microscope, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
Leica Microsystems dmi8 inverted fluorescent microscope
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Dmi8 Inverted Fluorescent Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Nikon inverted epifluorescence microscope eclipse ti-e
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Inverted Epifluorescence Microscope Eclipse Ti E, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evident Corporation ix83 epifluorescence microscope
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Ix83 Epifluorescence Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon microscope nikon te2000e2
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Microscope Nikon Te2000e2, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Carl Zeiss axio observer microscope
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Axio Observer Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss plan-apochromat 63x/1.4na oil objective
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Plan Apochromat 63x/1.4na Oil Objective, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon eclipse 80i epifluorescence microscope
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Eclipse 80i Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Carl Zeiss epifluorescent microscope
a) <t>Epifluorescent</t> and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.
Epifluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Nikon te2000 epifluorescence microscope
a) <t>Epifluorescent</t> and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.
Te2000 Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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86
Danaher Inc epifluorescent leica dmi8 afc microscope
a) <t>Epifluorescent</t> and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.
Epifluorescent Leica Dmi8 Afc Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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90
Evident Corporation ix81 inverted epifluorescence microscope
a) <t>Epifluorescent</t> and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.
Ix81 Inverted Epifluorescence Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ix81 inverted epifluorescence microscope/product/Evident Corporation
Average 90 stars, based on 1 article reviews
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Image Search Results


KEY RESOURCES TABLE

Journal: Neuron

Article Title: CD49f is a novel marker of functional and reactive human iPSC-derived astrocytes

doi: 10.1016/j.neuron.2020.05.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse neurons were treated with 70% ACM with 5μM IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius; 4440) and imaged every 6 hours for 60 hours on an Incucyte S3 epifluorescence time lapse microscope (Sartorius).

Techniques: Purification, Produced, Recombinant, Marker, Glutamate Assay, Imaging, Apoptosis Assay, Labeling, RNA Sequencing Assay, Transgenic Assay, Expressing, Software, Luminex, Western Blot

a) Epifluorescent and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.

Journal: bioRxiv

Article Title: Differential Dynamics and Roles of FKBP51 Isoforms and Their Implications for Targeted Therapies

doi: 10.1101/2024.08.03.606475

Figure Lengend Snippet: a) Epifluorescent and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.

Article Snippet: 24 hours after transfection, cells were live imaged with a Zeiss epifluorescent microscope.

Techniques: Imaging, Transfection, Control, Plasmid Preparation, Reporter Gene Assay, Comparison, Western Blot, MANN-WHITNEY