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Nikon
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Carl Zeiss
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Danaher Inc
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Image Search Results
Journal: Neuron
Article Title: CD49f is a novel marker of functional and reactive human iPSC-derived astrocytes
doi: 10.1016/j.neuron.2020.05.014
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Mouse neurons were treated with 70% ACM with 5μM IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius; 4440) and imaged every 6 hours for 60 hours on an
Techniques: Purification, Produced, Recombinant, Marker, Glutamate Assay, Imaging, Apoptosis Assay, Labeling, RNA Sequencing Assay, Transgenic Assay, Expressing, Software, Luminex, Western Blot
Journal: bioRxiv
Article Title: Differential Dynamics and Roles of FKBP51 Isoforms and Their Implications for Targeted Therapies
doi: 10.1101/2024.08.03.606475
Figure Lengend Snippet: a) Epifluorescent and bright field imaging of HeLa cells transfected with GFP-tagged FKBP51 isoform 1, GFP-tagged FKBP51 isoform 2, or GFP-control vector 24 hours prior to imaging. b-c) GRE-driven reporter gene assay performed in HeLa cells transfected with b) FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector (ctr vector), or c) in WT, full KO and Isoform 1 KO (iso1 KO) HeLa cells treated with 0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM or vehicle for 4 hours. Two-way ANOVA (shown in the box) with Tukey multiple comparisons test (shown in the graph). * indicates comparison with control/WT and isoform 1/full KO, # indicates comparison between isoform 1 and isoform 2, and $ refers to comparison between WT and iso 1 KO. *P < 0.05, **P < 0.01, ***P < 0.0005, ****P < 0.0001. d-h) Quantification of western blots analyses for different pathway markers from HeLa cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector: d) phosphorylated AKT (pAKT) normalised on total AKT, e-g) autophagy markers, BECN1, ATG12 and LC3BII/I; h) phosphorylated DNMT (pDNMT) normalised on total DNMT; i) Quantification of western blots analyses for phosphorilated NFAT (pNFAT) normalised on total NFAT from Jurkat cells transfected with FKBP51 isoform 1, FKBP51 isoform 2 or an empty vector; *P < 0.05. Mann-Whitney test. * and # indicate comparisons with ctr vector and isoform 2 respectively. Data shown as mean ± s.e.m.
Article Snippet: 24 hours after transfection, cells were live imaged with a
Techniques: Imaging, Transfection, Control, Plasmid Preparation, Reporter Gene Assay, Comparison, Western Blot, MANN-WHITNEY